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Nanoparticle Imaging In
Hyperspectral Light Scatter

Nanoparticle Imaging in Hyperspectral Light-Scatter maps the spatial distribution of Nanoparticles, Nanowires, and Nanorods in a Heterogeneous Field of View

  • The PARISS® Analytical Hyperspectral Imaging system: is a microscope accessory for nanoparticle imaging, spectral segmentation, and mapping
  • Live cells and tissue: Image single or thousands of nanoparticles, nanowires or nanorods in scattered light internalized in live, or prepared samples.
  • NON DESTRUCTIVE! Benign illumination keeps live cells alive!
  • No vacuum: Or special prep needed! Works with "wet" samples !
  • Reveal the location: nano sized objects can be imaged and mapped, in color-codes, onto a grayscale image of the field of view - with absolute accuracy!
  • Detect, analyze and characterize: Individual Nanoparticles imaged in scattered light can be analyzed in clumps, or aggregates in a heterogeneous field of view
  • Work in LOW magnification: Observe a huge field of view in magnifications ranging from 10 to 100X
  • Spectral identification of metals: Reveal the location of any material, especially metals, as a function of its spectrum in hyperspectral images
  • Reveal size, shape and proximity: Spectral images in dark-field scatter often correlates with size shape and proximity especially in the presence of surface plasmon resonance (SPR)
  • Low cost alternative: To SEM and TEM for many applications!
  • Data is instrument independent
  • Fluorescence: PARISS easily switches between light scatter and fluorescence
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Nanoparticle Imaging in living cells
Nanoparticle imaging a gold nanowire
Nanoparticle Imaging presentation
Figure 1: Nanoparticle Imaging:
Bio conjugated Au nanoparticles internalized within living cells in dark field
Figure 2: Gold/Silver nanowire reveals subtle differences indicated by pseudo-color spectral classification codes

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Nanoparticle Imaging in Hyperspectral
Light Scatter
is an effective means to obtain
High contrast and High Spectral Resolution

 
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Nanoparticle imaging with hyperspectral imaging